Immunofluorescent analysis of CXCR1/IL8RA (green) in MCF7 cells. Cells were blocked with blocking buffer (Product # 37525) for 30 min at room temparture and then stained with CXCR1/IL8RA mouse monoclonal antibody (Product # MA1-206) at a dilution of 1:100 for 1 hour at room temperature. After washing with PBS for 3 times, the cells were then stained with Alexa Fluor 488 goat anti-mouse secondary antibody (Product # A28175) for 1 hour at room temperature. The cells were fixed with 4% paraformaldehyde for 30 minutes at room temperature. Nuclei (blue) were counterstained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific EVOS FL Cell Imaging System at 40X magnification.